Hotspot

ycf1

SSC · 125635-131316 · 5682 bp

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Pi 0.0229

MarkerSeek score 64.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5682
variable_sites: 1164
pi: 0.0229
unique_haplotype_count: 88
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCAAGGTTTCAGACGAAA	CCCGAGTTTGGCAACTTTTT	45.0	45.0	58.0	57.4	1494-1542 bp	0.968421	82.7
ycf1_p2	ATGCCAAGGTTTCAGACGAA	CCCGAGTTTGGCAACTTTTT	45.0	45.0	57.7	57.4	1495-1543 bp	0.968421	80.9
ycf1_p3	ATTCGGTCCAAAAAGAGGGG	CCCGAGTTTGGCAACTTTTT	50.0	45.0	57.8	57.4	1020-1053 bp	0.968421	80.7
ycf1_p4	TCTTTGAGCACGTACAGAGC	CCCGAGTTTGGCAACTTTTT	50.0	45.0	57.9	57.4	955-988 bp	0.915789	79.8
ycf1_p5	CGTCTTTGAGCACGTACAGA	CCCGAGTTTGGCAACTTTTT	50.0	45.0	58.0	57.4	957-990 bp	0.821053	76.2