Hotspot

ndhF-rpl32

SSC · 113893-115018 · 1126 bp

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Pi 0.0314
MarkerSeek score 72.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1126
variable_sites
339
pi
0.0314
unique_haplotype_count
80
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAATGAACGGTATGATCCATG GAACTAGAGGTACGCTTTTT 45.5 40.0 57.0 53.0 1040-1326 bp 0.978947 51.2
ndhF-rpl32_p2 GGAATGAACGGTATGATCCA GAACTAGAGGTACGCTTTTT 45.0 40.0 54.9 53.0 1040-1326 bp 0.978947 51.2
ndhF-rpl32_p3 GGAATGAACGGTATGATCCATGA GAACTAGAGGTACGCTTTTT 43.5 40.0 58.4 53.0 1040-1326 bp 0.978947 51.2
ndhF-rpl32_p4 GGAATGAACGGTATGATCCAT GAACTAGAGGTACGCTTTTT 42.9 40.0 55.5 53.0 1040-1326 bp 0.978947 51.2
ndhF-rpl32_p5 TGAACGGTATGATCCATGAA GAACTAGAGGTACGCTTTTT 40.0 40.0 54.2 53.0 1036-1322 bp 0.978947 51.2