Hotspot

trnP-UGG-psaJ

LSC · 67681-68115 · 435 bp

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Pi 0.0365

MarkerSeek score 70.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 115
pi: 0.0365
unique_haplotype_count: 53
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCGAACCCGTGACATTTTGT	AGTACTTACTACCGGTGCCA	45.0	50.0	58.0	57.8	523-585 bp	1.0	89.9
trnP-UGG-psaJ_p2	GGATGACAGGATTCGAACCC	AGTACTTACTACCGGTGCCA	55.0	50.0	58.0	57.8	535-597 bp	1.0	89.9
trnP-UGG-psaJ_p3	GGGATGACAGGATTCGAACC	AGTACTTACTACCGGTGCCA	55.0	50.0	58.0	57.8	536-598 bp	1.0	89.9
trnP-UGG-psaJ_p4	ACCCGTGACATTTTGTACCC	AGTACTTACTACCGGTGCCA	50.0	50.0	58.1	57.8	519-581 bp	1.0	89.4
trnP-UGG-psaJ_p5	CCCGTGACATTTTGTACCCA	AGTACTTACTACCGGTGCCA	50.0	50.0	58.1	57.8	518-580 bp	1.0	89.4