Hotspot

psbE-petL

LSC · 65676-66893 · 1218 bp

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Pi 0.0247

MarkerSeek score 73.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 288
pi: 0.0247
unique_haplotype_count: 82
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GGCCGATAAATAGAGCTGAGG	50.0	52.4	57.8	58.0	974-1396 bp	1.0	82.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GGCCGATAAATAGAGCTGAGG	55.0	52.4	58.2	58.0	969-1391 bp	1.0	82.1
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GGCCGATAAATAGAGCTGAGG	50.0	52.4	58.4	58.0	967-1389 bp	1.0	80.9
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	GGCCGATAAATAGAGCTGAGG	50.0	52.4	58.6	58.0	968-1390 bp	1.0	79.1
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	GGCCGATAAATAGAGCTGAGG	45.0	52.4	57.4	58.0	975-1397 bp	1.0	78.8