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petA-psbJ

LSC · 63818-64847 · 1030 bp

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Pi 0.0336

MarkerSeek score 71.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 286
pi: 0.0336
unique_haplotype_count: 80
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCTCTTCTTGGCATCCGTTA	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1220-1568 bp	0.684211	77.5
petA-psbJ_p2	GCTCTTCTTGGCATCCGTTA	AACAGGCCGTTTTGACTCTT	50.0	45.0	58.0	57.9	1429-1777 bp	0.684211	76.8
petA-psbJ_p3	GCTCTTCTTGGCATCCGTTA	ACAGGCCGTTTTGACTCTTT	50.0	45.0	58.0	57.9	1428-1776 bp	0.684211	76.8
petA-psbJ_p4	GCTCTTCTTGGCATCCGTTA	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1313-1661 bp	0.589474	73.2
petA-psbJ_p5	GCTCTTCTTGGCATCCGTTA	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1311-1659 bp	0.589474	73.2