Hotspot

ndhC-trnV-UAC

LSC · 51924-52503 · 580 bp

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Pi 0.0399
MarkerSeek score 73.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
580
variable_sites
194
pi
0.0399
unique_haplotype_count
66
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACTATCAGAAATGCCCAGAA AGAAGGTCTACGGTTCGAGT 40.0 50.0 54.0 57.8 581-768 bp 0.978947 57.6
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAGAAGCA AGAAGGTCTACGGTTCGAGT 36.4 50.0 55.9 57.8 557-744 bp 0.978947 56.9
ndhC-trnV-UAC_p3 ACTATCAGAAATGCCCAGAAA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.9 57.8 581-768 bp 0.978947 56.7
ndhC-trnV-UAC_p4 ACTATCAGAAATGCCCAGAAAA AGAAGGTCTACGGTTCGAGT 36.4 50.0 55.7 57.8 581-768 bp 0.978947 55.1
ndhC-trnV-UAC_p5 ACTATCAGAAATGCCCAGAA GAAGGTCTACGGTTCGAGTC 40.0 55.0 54.0 57.5 580-767 bp 0.978947 54.9