Hotspot

ycf3-trnS-GGA

LSC · 45340-46046 · 707 bp

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Pi 0.0244
MarkerSeek score 73.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
707
variable_sites
173
pi
0.0244
unique_haplotype_count
70
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 552-1095 bp 0.947368 76.0
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 553-1096 bp 0.947368 76.0
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 502-1045 bp 0.947368 75.7
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 503-1046 bp 0.947368 75.7
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 504-1047 bp 0.947368 75.1