Hotspot

rpoB-trnC-GCA

LSC · 26396-27558 · 1163 bp

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Pi 0.0243

MarkerSeek score 72.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 281
pi: 0.0243
unique_haplotype_count: 70
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GACTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.2	58.1	1121-1280 bp	0.989474	89.6
rpoB-trnC-GCA_p2	ATTGACTCATTTCCACCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1124-1283 bp	0.989474	86.7
rpoB-trnC-GCA_p3	TGACTCATTTCCACCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.8	58.1	1122-1281 bp	0.989474	84.2
rpoB-trnC-GCA_p4	GACTCATTTCCACCCCCAAG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.2	58.4	1103-1262 bp	0.905263	84.1
rpoB-trnC-GCA_p5	ATTGACTCATTTCCACCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.5	58.4	1106-1265 bp	0.905263	81.1