Hotspot

atpF-atpH

LSC · 12869-13387 · 519 bp

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Pi 0.0277

MarkerSeek score 72.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 136
pi: 0.0277
unique_haplotype_count: 68
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCCG	CGGAAGGAAAAATACGGGGT	50.0	50.0	57.9	57.9	684-749 bp	0.947368	88.0
atpF-atpH_p2	ATTAAACCCGAAACTCCCCG	CCAGAGGCGGAAGGAAAAAT	50.0	50.0	57.9	58.2	691-756 bp	0.947368	87.7
atpF-atpH_p3	ATTAAACCCGAAACTCCCCG	AGGCGGAAGGAAAAATACGG	50.0	50.0	57.9	58.3	687-752 bp	0.947368	86.9
atpF-atpH_p4	GATGGCCAATAACCCAAGGA	CGGAAGGAAAAATACGGGGT	50.0	50.0	57.5	57.9	611-727 bp	0.957895	85.7
atpF-atpH_p5	GATGGCCAATAACCCAAGGA	CCAGAGGCGGAAGGAAAAAT	50.0	50.0	57.5	58.2	618-734 bp	0.957895	85.4