Hotspot

rps16-trnQ-UUG

LSC · 6331-7368 · 1038 bp

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Pi 0.0331
MarkerSeek score 77.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1038
variable_sites
347
pi
0.0331
unique_haplotype_count
79
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 796-1224 bp 1.0 91.2
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 794-1222 bp 1.0 91.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 778-1206 bp 0.989474 89.9
rps16-trnQ-UUG_p4 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 820-1238 bp 0.947368 88.7
rps16-trnQ-UUG_p5 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 821-1239 bp 0.947368 88.7