Hotspot

atpF

LSC · 12390-13972 · 1583 bp

Back to Potamogeton

Pi 0.00777

MarkerSeek score 53.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1583
variable_sites: 24
pi: 0.00777
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAGAGCAATACCCACCGTAC	GTATTGCGAGACAGCCAGAA	55.0	50.0	58.1	58.0	2119-2149 bp	1.0	82.6
atpF_p2	TCCGTTGCTACTGCTGTTTT	GTATTGCGAGACAGCCAGAA	45.0	50.0	58.0	58.0	2469-2499 bp	1.0	82.5
atpF_p3	ATCCCCTACCTGAAGTACGG	GTATTGCGAGACAGCCAGAA	55.0	50.0	57.9	58.0	2035-2065 bp	1.0	82.5
atpF_p4	CAGAGCAATACCCACCGTAC	TGGATTGGTCGTGGCATTAG	55.0	50.0	58.1	58.0	2034-2064 bp	1.0	82.5
atpF_p5	TCCGTTGCTACTGCTGTTTT	TGGATTGGTCGTGGCATTAG	45.0	50.0	58.0	58.0	2384-2414 bp	1.0	82.4