Hotspot

ndhG-ndhI

SSC · 120682-121232 · 551 bp

Back to Potamogeton

Pi 0.0129
MarkerSeek score 67.90
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
551
variable_sites
15
pi
0.0129
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAACTACTCCCAGACTTCCT GGAGGATTACACAATTCAAGCA 45.0 40.9 55.1 56.7 724-760 bp 1.0 43.8
ndhG-ndhI_p2 AAACTACTCCCAGACTTCCT TGGAGGATTACACAATTCAAGC 45.0 40.9 55.1 56.7 725-761 bp 1.0 43.8
ndhG-ndhI_p3 AAACTACTCCCAGACTTCCT TGGAGGATTACACAATTCAAGCA 45.0 39.1 55.1 58.3 725-761 bp 1.0 43.8
ndhG-ndhI_p4 AAAACTACTCCCAGACTTCCT GGAGGATTACACAATTCAAGCA 42.9 40.9 55.9 56.7 725-761 bp 1.0 43.8
ndhG-ndhI_p5 AAAACTACTCCCAGACTTCCT TGGAGGATTACACAATTCAAGC 42.9 40.9 55.9 56.7 726-762 bp 1.0 43.8