Hotspot

ndhD-psaC

SSC · 118800-118925 · 126 bp

Back to Potamogeton

Pi 0.0413

MarkerSeek score 66.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 126
variable_sites: 13
pi: 0.0413
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAACCTGCAAATATCGGCA	AGACAACCCGTAGTATGGCT	45.0	50.0	57.6	58.1	215 bp	1.0	80.4
ndhD-psaC_p2	AGAACCTGCAAATATCGGCA	TTTATGGCACGAGACAACCC	45.0	50.0	57.6	58.2	226 bp	1.0	79.8
ndhD-psaC_p3	AGAACCTGCAAATATCGGCA	GAGACAACCCGTAGTATGGC	45.0	55.0	57.6	57.8	216 bp	1.0	79.7
ndhD-psaC_p4	AGAACCTGCAAATATCGGCA	AAGAGATGTGAAACCGCCTG	45.0	50.0	57.6	58.2	276 bp	1.0	78.9
ndhD-psaC_p5	AGAACCTGCAAATATCGGCA	AGAGGACTGCGTAGGTTGTA	45.0	50.0	57.6	57.8	295 bp	1.0	78.5