Hotspot

ndhF-rpl32

SSC · 114186-115161 · 976 bp

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Pi 0.0143

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 976
variable_sites: 28
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACACACTCCCACCAATTCC	AGCTTTAACCGCCCACAAAT	50.0	45.0	58.0	58.4	1517-1578 bp	1.0	81.4
ndhF-rpl32_p2	GGAACACACTCCCACCAATT	AGCTTTAACCGCCCACAAAT	50.0	45.0	58.0	58.4	1519-1580 bp	1.0	81.4
ndhF-rpl32_p3	CACTCCCACCAATTCCCAAA	AGCTTTAACCGCCCACAAAT	50.0	45.0	58.0	58.4	1513-1574 bp	1.0	81.4
ndhF-rpl32_p4	CTAATCCCAACATGGACGCA	AGCTTTAACCGCCCACAAAT	50.0	45.0	58.0	58.4	1460-1521 bp	1.0	81.2
ndhF-rpl32_p5	ACTAATCCCAACATGGACGC	AGCTTTAACCGCCCACAAAT	50.0	45.0	58.0	58.4	1461-1522 bp	1.0	81.2