Hotspot

petD-rpoA

LSC · 79594-79774 · 181 bp

Back to Potamogeton

Pi 0.02

MarkerSeek score 60.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 7
pi: 0.02
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CCGCAGTAGCACTTTGGTTA	TCGCACGGAAGATGTAAACC	50.0	50.0	58.2	58.3	332-340 bp	1.0	79.7
petD-rpoA_p2	TTGGTTAGGCATTGGAGCAA	TCGCACGGAAGATGTAAACC	45.0	50.0	57.7	58.3	319-327 bp	1.0	79.0
petD-rpoA_p3	TTTGGTTAGGCATTGGAGCA	TCGCACGGAAGATGTAAACC	45.0	50.0	57.7	58.3	320-328 bp	1.0	79.0
petD-rpoA_p4	CCGTCCTGTAGCTACAACAG	TCGCACGGAAGATGTAAACC	55.0	50.0	57.7	58.3	367-375 bp	1.0	78.7
petD-rpoA_p5	GCACTTTGGTTAGGCATTGG	TCGCACGGAAGATGTAAACC	50.0	50.0	57.6	58.3	324-332 bp	1.0	78.4