Hotspot

trnF-GAA-ndhJ

LSC · 49553-50192 · 640 bp

Back to Potamogeton

Pi 0.0113

MarkerSeek score 63.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 14
pi: 0.0113
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	58.1	57.9	735-762 bp	1.0	82.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGGCTGGCCTTTACGTAAAG	50.0	50.0	58.1	57.9	736-763 bp	1.0	82.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	748-775 bp	1.0	82.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	779-806 bp	1.0	79.8
trnF-GAA-ndhJ_p5	TCGGGATAGCTCAGTTGGTA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	57.6	57.9	773-800 bp	1.0	79.0