Hotspot

trnT-GGU-psbD

LSC · 33108-34170 · 1063 bp

Back to Potamogeton

Pi 0.012

MarkerSeek score 61.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 26
pi: 0.012
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AATAAACCGGACCAACCCAC	55.0	50.0	58.1	58.1	1199-1231 bp	1.0	83.0
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CAATAAACCGGACCAACCCA	55.0	50.0	58.1	58.1	1200-1232 bp	1.0	83.0
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	AATAAACCGGACCAACCCAC	50.0	50.0	58.1	58.1	1202-1234 bp	1.0	82.8
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CAATAAACCGGACCAACCCA	50.0	50.0	58.1	58.1	1203-1235 bp	1.0	82.8
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	55.0	50.0	58.1	58.2	1182-1214 bp	1.0	82.4