Hotspot

trnE-UUC-trnT-GGU

LSC · 32518-33035 · 518 bp

Back to Potamogeton

Pi 0.0141
MarkerSeek score 62.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
518
variable_sites
16
pi
0.0141
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 601-631 bp 1.0 84.0
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 599-629 bp 1.0 84.0
trnE-UUC-trnT-GGU_p3 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 578-608 bp 1.0 83.8
trnE-UUC-trnT-GGU_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 580-610 bp 1.0 83.7
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 655-685 bp 1.0 83.5