Hotspot

petN-psbM

LSC · 29652-30690 · 1039 bp

Back to Potamogeton

Pi 0.0177

MarkerSeek score 73.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 34
pi: 0.0177
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TTGTAGTGTGGGGAAGGAGT	TCTAGTTCCTACCGCTTTTT	50.0	40.0	57.9	54.0	996-1129 bp	1.0	53.7
petN-psbM_p2	TTGTAGTGTGGGGAAGGAGT	TTCTAGTTCCTACCGCTTTT	50.0	40.0	57.9	54.0	997-1130 bp	1.0	53.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TCTAGTTCCTACCGCTTTTT	50.0	40.0	58.2	54.0	1050-1183 bp	1.0	53.3
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTCTAGTTCCTACCGCTTTT	50.0	40.0	58.2	54.0	1051-1184 bp	1.0	53.3
petN-psbM_p5	TCCCTTTCGCTTGTAGTGTG	TCTAGTTCCTACCGCTTTTT	50.0	40.0	57.8	54.0	1006-1139 bp	1.0	53.3