Hotspot

rps16-trnQ-UUG

LSC · 5817-7034 · 1218 bp

Back to Potamogeton

Pi 0.0178

MarkerSeek score 62.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 45
pi: 0.0178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCCAGACAAGTTGGGATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1401-1439 bp	1.0	83.9
rps16-trnQ-UUG_p2	TGATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1302-1340 bp	1.0	83.0
rps16-trnQ-UUG_p3	CGTTGCTTTCTTCCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1283-1321 bp	1.0	82.8
rps16-trnQ-UUG_p4	GAGCTCCATCGTGCACTATT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1432-1470 bp	1.0	82.3
rps16-trnQ-UUG_p5	AACTATGTCGAGCCAAGAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1368-1406 bp	1.0	81.9