Hotspot

ycf1

IRb · 108974-114736 · 5763 bp

Back to Portulaca

Pi 0.0325

MarkerSeek score 45.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5763
variable_sites: 83
pi: 0.0325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.31
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCCTTTTGGTTCTCCCCG	CCAGATTCGATATGGGACGAC	50.0	52.4	58.0	58.1	1918 bp	0.333333	44.8
ycf1_p2	GGGGAATGGAAACCGACTTT	CCAGATTCGATATGGGACGAC	50.0	52.4	58.1	58.1	1935 bp	0.333333	44.3
ycf1_p3	TTTCCTTTTGGTTCTCCCCG	TCCAGATTCGATATGGGACGA	50.0	47.6	58.0	58.1	1919 bp	0.333333	44.2
ycf1_p4	GGATACGACCTTCTCATGGC	CCAGATTCGATATGGGACGAC	55.0	52.4	57.8	58.1	1998 bp	0.333333	43.7
ycf1_p5	GGGGAATGGAAACCGACTTT	TCCAGATTCGATATGGGACGA	50.0	47.6	58.1	58.1	1936 bp	0.333333	43.7