Hotspot

rpl22

LSC · 85040-85744 · 705 bp

Back to Portulaca

Pi 0.117

MarkerSeek score 80.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 705
variable_sites: 121
pi: 0.117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGAACCGTATAGGCGCAATA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	57.9	58.0	1960-1972 bp	1.0	86.3
rpl22_p2	AATTTTCGCGCGAATGGTTT	TGGGTAGAGCCGGATCTAAG	40.0	55.0	57.9	58.0	1937-1949 bp	1.0	86.3
rpl22_p3	CGAACCGTATAGGCGCAATA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.9	58.1	1861-1873 bp	1.0	86.2
rpl22_p4	CGAACCGTATAGGCGCAATA	GAGGGTAAATCGGCCACATT	50.0	50.0	57.9	57.9	2072-2084 bp	1.0	85.6
rpl22_p5	AATTTTCGCGCGAATGGTTT	GAGGGTAAATCGGCCACATT	40.0	50.0	57.9	57.9	2049-2061 bp	1.0	85.6