Hotspot

clpP

LSC · 72686-73285 · 600 bp

Back to Portulaca

Pi 0.149

MarkerSeek score 79.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 600
variable_sites: 131
pi: 0.149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TAACGTTCGCTACCAATGGG	AGTAGCAATACGCAATGGGG	50.0	50.0	58.0	58.0	1318-2999 bp	1.0	88.7
clpP_p2	TAACGTTCGCTACCAATGGG	GTAGCAATACGCAATGGGGA	50.0	50.0	58.0	58.0	1317-2998 bp	1.0	88.6
clpP_p3	TAACGTTCGCTACCAATGGG	ATGCAATGGACCCCTTTTGT	50.0	45.0	58.0	58.0	1439-2912 bp	0.666667	74.7
clpP_p4	TAACGTTCGCTACCAATGGG	ATACCAAGGCAAACCCATGG	50.0	50.0	58.0	58.1	1524-2997 bp	0.666667	73.5
clpP_p5	TAACGTTCGCTACCAATGGG	ACGCAATGGGGAGTTGATAC	50.0	50.0	58.0	58.0	1309-2990 bp	0.666667	72.8