Hotspot

trnI-CAU-trnH-GUG

IRa · 155582-156125 · 544 bp

Back to Portulaca

Pi 0.151

MarkerSeek score 76.00

Primer available yes

Label trnI-CAU-trnH-GUG

Feature Metadata

label_name: trnI-CAU-trnH-GUG
feature_type: igs
region: IRa
length_bp: 544
variable_sites: 86
pi: 0.151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnH-GUG_p1	CAACGGGACCCTCCAATAAG	AGAAGAGTTCGACCCAATGC	55.0	50.0	58.0	57.9	289-471 bp	1.0	90.9
trnI-CAU-trnH-GUG_p2	CAACGGGACCCTCCAATAAG	GAAGAGTTCGACCCAATGCT	55.0	50.0	58.0	57.9	288-470 bp	1.0	90.9
trnI-CAU-trnH-GUG_p3	AACGGGACCCTCCAATAAGT	AGAAGAGTTCGACCCAATGC	50.0	50.0	58.3	57.9	288-470 bp	1.0	88.5
trnI-CAU-trnH-GUG_p4	AACGGGACCCTCCAATAAGT	GAAGAGTTCGACCCAATGCT	50.0	50.0	58.3	57.9	287-469 bp	1.0	88.5
trnI-CAU-trnH-GUG_p5	CAACGGGACCCTCCAATAAG	CCAAAGAAGAGTTCGACCCA	55.0	50.0	58.0	57.5	293-475 bp	1.0	87.3