Hotspot

ycf1-trnN-GUU

IRa · 134237-134582 · 346 bp

Back to Portulaca

Pi 0.0819

MarkerSeek score 79.20

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 346
variable_sites: 37
pi: 0.0819
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	CCTGTGGAGAATGTGGTCAG	GGCAGAATAGCAGAGCAAGT	55.0	50.0	57.9	58.0	873-1353 bp	1.0	86.1
ycf1-trnN-GUU_p2	CCTGTGGAGAATGTGGTCAG	GAACTTGGGTGTGGGTCTTT	55.0	50.0	57.9	57.9	1025-1515 bp	1.0	85.9
ycf1-trnN-GUU_p3	AGGAAGAGATAAGAGGGCCC	GGCAGAATAGCAGAGCAAGT	55.0	50.0	57.9	58.0	891-926 bp	0.666667	70.6
ycf1-trnN-GUU_p4	GGAAGAGATAAGAGGGCCCT	GGCAGAATAGCAGAGCAAGT	55.0	50.0	57.9	58.0	890-925 bp	0.666667	70.6
ycf1-trnN-GUU_p5	TTCCATAACCAGAGCTCGGA	GGCAGAATAGCAGAGCAAGT	50.0	50.0	58.1	58.0	913-948 bp	0.666667	70.3