Hotspot

rpl32-ccsA

SSC · 118688-119020 · 333 bp

Back to Portulaca

Pi 0.136
MarkerSeek score 76.90
Primer available yes
Label rpl32-ccsA

Feature Metadata

label_name
rpl32-ccsA
feature_type
igs
region
SSC
length_bp
333
variable_sites
45
pi
0.136
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ccsA_p1 GGGATATTGGGCAGCGTTAA ATCCGCATAAAAGAGCTGCA 50.0 45.0 58.0 58.0 899-1640 bp 0.666667 69.7
rpl32-ccsA_p2 GGGATATTGGGCAGCGTTAA TCCGCATAAAAGAGCTGCAT 50.0 45.0 58.0 58.0 898-1639 bp 0.666667 69.7
rpl32-ccsA_p3 GGGCAGCGTTAAAAGCTTTT TCCGCATAAAAGAGCTGCAT 45.0 45.0 57.9 58.0 890-1631 bp 0.666667 68.8
rpl32-ccsA_p4 GGGATATTGGGCAGCGTTAA CGAAAGGGGAGAAGCACTTT 50.0 50.0 58.0 58.1 1060 bp 0.333333 52.3
rpl32-ccsA_p5 GGGATATTGGGCAGCGTTAA GAAACGAAAGGGGAGAAGCA 50.0 50.0 58.0 58.1 1064 bp 0.333333 52.3