Hotspot

ndhF-rpl32

SSC · 117259-118513 · 1255 bp

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Pi 0.0526

MarkerSeek score 77.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1255
variable_sites: 82
pi: 0.0526
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCATATACGCCGAAGATT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.9	58.0	1449-1465 bp	0.666667	72.7
ndhF-rpl32_p2	GCCAAAATCCCCTACACGAT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.9	58.0	1891-1907 bp	0.666667	71.7
ndhF-rpl32_p3	ACAAACGCTTTTTGACAGGC	TTAACGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	1866-1882 bp	0.666667	71.5
ndhF-rpl32_p4	CAAACGCTTTTTGACAGGCA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	1865-1881 bp	0.666667	71.5
ndhF-rpl32_p5	ACATAGGAACCGGAAGTGGA	TTAACGCTGCCCAATATCCC	50.0	50.0	58.1	58.0	1404 bp	0.333333	52.7