Hotspot

rpl2-trnI-CAU

IRb · 86985-87628 · 644 bp

Back to Portulaca

Pi 0.143

MarkerSeek score 78.20

Primer available yes

Label rpl2-trnI-CAU

Feature Metadata

label_name: rpl2-trnI-CAU
feature_type: igs
region: IRb
length_bp: 644
variable_sites: 99
pi: 0.143
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnI-CAU_p1	AATGTGGCCGATTTACCCTC	GAATGGTTAAAGCGCCCAAC	50.0	50.0	57.9	58.0	1042-1239 bp	1.0	91.0
rpl2-trnI-CAU_p2	CTTAGATCCGGCTCTACCCA	GAATGGTTAAAGCGCCCAAC	55.0	50.0	58.0	58.0	1154-1351 bp	1.0	90.8
rpl2-trnI-CAU_p3	CTTAGATCCGGCTCTACCCA	ATTCACCATGCACGAGGATC	55.0	50.0	58.0	58.0	1194-1391 bp	1.0	90.4
rpl2-trnI-CAU_p4	CTTAGATCCGGCTCTACCCA	GGAGCGAAACAATCAGCCTA	55.0	50.0	58.0	58.0	1677-1926 bp	1.0	90.3
rpl2-trnI-CAU_p5	TTTTCTACCAATTGGGGCCC	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.1	58.0	1253-1450 bp	1.0	89.9