Hotspot

trnR-UCU-atpA

LSC · 9595-9746 · 152 bp

Back to Portulaca

Pi 0.0671

MarkerSeek score 75.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 15
pi: 0.0671
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.0	58.1	480-483 bp	1.0	83.3
trnR-UCU-atpA_p2	AGCCTTCCAAGCTAACGATG	GGGATCTGCGGCTCAAATTA	50.0	50.0	58.0	58.0	829-832 bp	1.0	83.0
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	TAAACAACCCCAATCTGCCC	50.0	50.0	58.0	58.1	667-670 bp	1.0	83.0
trnR-UCU-atpA_p4	CCTAGCCTTCCAAGCTAACG	TAAACAACCCCAATCTGCCC	55.0	50.0	58.1	58.1	670-673 bp	1.0	82.8
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	GGGATCTGCGGCTCAAATTA	55.0	50.0	58.1	58.0	832-835 bp	1.0	82.7