Hotspot

psbI-trnS-GCU

LSC · 7515-7699 · 185 bp

Back to Portulaca

Pi 0.11

MarkerSeek score 84.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 28
pi: 0.11
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGACGCGAAGAA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.7	58.6	291-313 bp	1.0	83.9
psbI-trnS-GCU_p2	GTAATCCTGGACGCGAAGAA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	57.7	57.0	228-250 bp	1.0	82.0
psbI-trnS-GCU_p3	GTAATCCTGGACGCGAAGAA	CGAAAGCGTCGGATTGCTAA	50.0	50.0	57.7	59.0	277-299 bp	1.0	81.4
psbI-trnS-GCU_p4	GTAATCCTGGACGCGAAGAA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	57.7	56.9	231-253 bp	1.0	81.0
psbI-trnS-GCU_p5	GTAATCCTGGACGCGAAGAA	AGATGGCTGAGTGGACGAAA	50.0	50.0	57.7	59.0	292-314 bp	1.0	80.8