Hotspot

rps16-trnQ-UUG

LSC · 5882-6379 · 498 bp

Back to Portulaca

Pi 0.058

MarkerSeek score 82.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 42
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	571-639 bp	1.0	85.5
rps16-trnQ-UUG_p2	CCACAACAGATCGTGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	592-660 bp	1.0	85.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	601-669 bp	1.0	84.4
rps16-trnQ-UUG_p4	CCACAACAGATCGTGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.8	58.2	622-690 bp	1.0	83.9
rps16-trnQ-UUG_p5	ATCCACAACAGATCGTGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	594-662 bp	1.0	83.2