Hotspot

trnH-GUG-psbA

LSC · 71-336 · 266 bp

Back to Portulaca

Pi 0.0855

MarkerSeek score 79.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 33
pi: 0.0855
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GGCCTGTAGTGGGTATTTGG	55.0	55.0	58.1	57.9	512-517 bp	1.0	86.3
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATATTCAGCTCCGGTTGCAG	55.0	50.0	58.1	58.0	907-912 bp	1.0	83.0
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	ATATTCAGCTCCGGTTGCAG	50.0	50.0	57.9	58.0	921-926 bp	1.0	82.9
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	ATATTCAGCTCCGGTTGCAG	50.0	50.0	57.9	58.0	920-925 bp	1.0	82.9
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	ATATTCAGCTCCGGTTGCAG	50.0	50.0	57.9	58.0	922-927 bp	1.0	82.9