Hotspot

syh

Genome · 137483-138775 · 1293 bp

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Pi 0.11

MarkerSeek score 78.90

Primer available yes

Label syh

Feature Metadata

label_name: syh
feature_type: gene
region: Genome
length_bp: 1293
variable_sites: 142
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syh_p1	CTAGGTCAGCGGTTCAAGTC	GGTAGACAATTACCTCCGCG	55.0	55.0	58.0	58.2	1851 bp	0.5	58.8
syh_p2	ATTTAACTGGGGTGGGAGGA	GGTAGACAATTACCTCCGCG	50.0	55.0	57.9	58.2	1794 bp	0.5	58.2
syh_p3	TTAACTGGGGTGGGAGGATT	GGTAGACAATTACCTCCGCG	50.0	55.0	57.9	58.2	1792 bp	0.5	58.2
syh_p4	TTTAACTGGGGTGGGAGGAT	GGTAGACAATTACCTCCGCG	50.0	55.0	57.9	58.2	1793 bp	0.5	58.2
syh_p5	AAGCGCAGACCGAAAGATAG	GGTAGACAATTACCTCCGCG	50.0	55.0	58.1	58.2	2221 bp	0.5	58.2