Hotspot

rne

Genome · 18128-19663 · 1536 bp

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Pi 0.0996

MarkerSeek score 78.90

Primer available yes

Label rne

Feature Metadata

label_name: rne
feature_type: gene
region: Genome
length_bp: 1536
variable_sites: 153
pi: 0.0996
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rne_p1	TGGTGGTGAACAAGTAACGG	ATTCCAAGCTGAGCACAACA	50.0	45.0	58.0	58.0	2540 bp	0.5	59.5
rne_p2	TGGTGGTGAACAAGTAACGG	TTCCAAGCTGAGCACAACAT	50.0	45.0	58.0	58.0	2539 bp	0.5	59.5
rne_p3	ACAAGGAATGGCCGAGATTC	ATTCCAAGCTGAGCACAACA	50.0	45.0	58.0	58.0	2590 bp	0.5	59.5
rne_p4	ACAAGGAATGGCCGAGATTC	TTCCAAGCTGAGCACAACAT	50.0	45.0	58.0	58.0	2589 bp	0.5	59.5
rne_p5	GGTAGTACAAGGAATGGCCG	TTCCAAGCTGAGCACAACAT	55.0	45.0	58.1	58.0	2595 bp	0.5	59.5