Hotspot

preA

Genome · 74450-75421 · 972 bp

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Pi 0.11

MarkerSeek score 79.60

Primer available yes

Label preA

Feature Metadata

label_name: preA
feature_type: gene
region: Genome
length_bp: 972
variable_sites: 107
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
preA_p1	ATGCATTGATCCACCTCGTC	CTGCAGCACATAGTCAAGGT	50.0	50.0	58.0	57.9	1813-1814 bp	1.0	87.1
preA_p2	ATGCATTGATCCACCTCGTC	TCTTCAAAACACAGACGGCA	50.0	45.0	58.0	58.0	2157 bp	0.5	59.4
preA_p3	ATGCATTGATCCACCTCGTC	GGCATCTTTCCTCCACATGT	50.0	50.0	58.0	57.9	2141 bp	0.5	58.6
preA_p4	ATGCATTGATCCACCTCGTC	AGATTTTCGTCGCCTTTGGA	50.0	45.0	58.0	57.8	1848 bp	0.5	58.2
preA_p5	ATGCATTGATCCACCTCGTC	TCAAAACACAGACGGCATCT	50.0	45.0	58.0	57.7	2154 bp	0.5	57.6