Hotspot

infB

Genome · 60495-62786 · 2292 bp

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Pi 0.108

MarkerSeek score 77.10

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2292
variable_sites: 248
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	GTGACAAACTTTTCACGTTACGA	TGTGCTGACATTCCATCTCG	39.1	50.0	58.1	58.0	2937 bp	0.5	35.0
infB_p2	TGACAAACTTTTCACGTTACGAG	TGTGCTGACATTCCATCTCG	39.1	50.0	57.9	58.0	2936 bp	0.5	34.8
infB_p3	TGTTTGTGACAAACTTTTCACGT	TGTGCTGACATTCCATCTCG	34.8	50.0	58.1	58.0	2942 bp	0.5	34.8
infB_p4	TGACAAACTTTTCACGTTACGA	TGTGCTGACATTCCATCTCG	36.4	50.0	56.8	58.0	2936 bp	0.5	34.4
infB_p5	GTGACAAACTTTTCACGTTACG	TGTGCTGACATTCCATCTCG	40.9	50.0	56.8	58.0	2937 bp	0.5	34.1