Hotspot

PopuC001

Genome · 132858-136455 · 3598 bp

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Pi 0.116

MarkerSeek score 75.40

Primer available yes

Label PopuC001

Feature Metadata

label_name: PopuC001
feature_type: gene
region: Genome
length_bp: 3598
variable_sites: 419
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
PopuC001_p1	ACACGGATATTTAGAATAAGGCTGA	TCAGCTACTTTAACTTATAACAATGA	36.0	26.9	57.9	54.8	189 bp	1.0	48.0
PopuC001_p2	ACACGGATATTTAGAATAAGGCTG	TCAGCTACTTTAACTTATAACAATGA	37.5	26.9	56.6	54.8	189 bp	1.0	48.0
PopuC001_p3	ACACGGATATTTAGAATAAGGCTGA	CAGCTACTTTAACTTATAACAATGA	36.0	28.0	57.9	53.4	188 bp	1.0	48.0
PopuC001_p4	ACACGGATATTTAGAATAAGGCTGA	TCAGCTACTTTAACTTATAACAATG	36.0	28.0	57.9	53.4	189 bp	1.0	48.0
PopuC001_p5	ACACGGATATTTAGAATAAGGCTGA	TCAGCTACTTTAACTTATAACAATGAT	36.0	25.9	57.9	55.3	189 bp	1.0	48.0