Hotspot

ORF621

Genome · 35775-37640 · 1866 bp

Back to Porphyra

Pi 0.164

MarkerSeek score 80.50

Primer available yes

Label ORF621

Feature Metadata

label_name: ORF621
feature_type: gene
region: Genome
length_bp: 1866
variable_sites: 266
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF621_p1	GATCTTGGGATGGGACCAAC	TCCCCACCTCAATGAAGCTA	55.0	50.0	57.9	58.0	2606 bp	0.5	59.3
ORF621_p2	GATCTTGGGATGGGACCAAC	GAGATCTCTCCCCACCTCAA	55.0	55.0	57.9	57.8	2614 bp	0.5	58.2
ORF621_p3	GATCTTGGGATGGGACCAAC	TATGTTGTGCGTTGCGAGAT	55.0	45.0	57.9	58.3	2503 bp	0.5	57.3
ORF621_p4	CTTGGGATGGGACCAACTTT	TCCCCACCTCAATGAAGCTA	50.0	50.0	57.7	58.0	2603 bp	0.5	57.3
ORF621_p5	ATCTTGGGATGGGACCAACT	TCCCCACCTCAATGAAGCTA	50.0	50.0	58.3	58.0	2605 bp	0.5	57.2