Hotspot

ORF450

Genome · 24455-25807 · 1353 bp

Back to Porphyra

Pi 0.16

MarkerSeek score 78.90

Primer available yes

Label ORF450

Feature Metadata

label_name: ORF450
feature_type: gene
region: Genome
length_bp: 1353
variable_sites: 216
pi: 0.16
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF450_p1	AATGGACGTGGGTTCAAGTC	ACCACGAGTACGCATTTCTC	50.0	50.0	58.1	58.0	2577-2586 bp	1.0	87.4
ORF450_p2	AAGCAAGGAGGTTGTTCAGG	ACCACGAGTACGCATTTCTC	50.0	50.0	58.0	58.0	2386 bp	0.5	59.8
ORF450_p3	AAGCAAGGAGGTTGTTCAGG	GACCACGAGTACGCATTTCT	50.0	50.0	58.0	58.0	2387 bp	0.5	59.8
ORF450_p4	GCAAGGAGGTTGTTCAGGAA	ACCACGAGTACGCATTTCTC	50.0	50.0	58.0	58.0	2384 bp	0.5	59.8
ORF450_p5	GCAAGGAGGTTGTTCAGGAA	GACCACGAGTACGCATTTCT	50.0	50.0	58.0	58.0	2385 bp	0.5	59.8