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ORF287

Genome · 39975-40838 · 864 bp

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Pi 0.132

MarkerSeek score 78.90

Primer available yes

Label ORF287

Feature Metadata

label_name: ORF287
feature_type: gene
region: Genome
length_bp: 864
variable_sites: 114
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF287_p1	CATGACCGAAGTCGTCACAT	GTTGCAATTGAGACGCTGTC	50.0	50.0	58.0	58.0	1572 bp	0.5	59.6
ORF287_p2	GAACCCTCATGACCGAAGTC	GTTGCAATTGAGACGCTGTC	55.0	50.0	58.0	58.0	1579 bp	0.5	59.6
ORF287_p3	TGCGTATACCAATTTCGCCA	GTTGCAATTGAGACGCTGTC	45.0	50.0	58.0	58.0	1541 bp	0.5	59.4
ORF287_p4	GGAATCGAACCCTCATGACC	GTTGCAATTGAGACGCTGTC	55.0	50.0	58.0	58.0	1585 bp	0.5	59.3
ORF287_p5	GGGAATCGAACCCTCATGAC	GTTGCAATTGAGACGCTGTC	55.0	50.0	58.0	58.0	1586 bp	0.5	59.3