Hotspot

ccs1-ORF240

Genome · 156595-156862 · 268 bp

Back to Porphyra

Pi 0.264

MarkerSeek score 75.10

Primer available yes

Label ccs1-ORF240

Feature Metadata

label_name: ccs1-ORF240
feature_type: igs
region: Genome
length_bp: 268
variable_sites: 69
pi: 0.264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1-ORF240_p1	ATAGCTGAATTGGCCGGAAG	CTATGGGCTCCATTTAGCGG	50.0	55.0	58.0	58.2	997 bp	0.5	58.3
ccs1-ORF240_p2	AAGCACCATCTTCTCGCATT	CTATGGGCTCCATTTAGCGG	45.0	55.0	57.9	58.2	693 bp	0.5	57.5
ccs1-ORF240_p3	AGCACCATCTTCTCGCATTT	CTATGGGCTCCATTTAGCGG	45.0	55.0	57.9	58.2	692 bp	0.5	57.5
ccs1-ORF240_p4	ATGCTTGCGTGCACAAAAAT	CTATGGGCTCCATTTAGCGG	40.0	55.0	58.1	58.2	876 bp	0.5	57.5
ccs1-ORF240_p5	TGAATTGGCCGGAAGCAATA	CTATGGGCTCCATTTAGCGG	45.0	55.0	57.9	58.2	992 bp	0.5	57.4