Hotspot

trnR_3-groEL

Genome · 138954-139947 · 994 bp

Back to Porphyra

Pi 0.238

MarkerSeek score 68.70

Primer available yes

Label trnR_3-groEL

Feature Metadata

label_name: trnR_3-groEL
feature_type: igs
region: Genome
length_bp: 994
variable_sites: 70
pi: 0.238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.30
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR_3-groEL_p1	AATCCACTGAGCTACAAGCC	GCATCATCTTGATATAGAATTTGT	50.0	29.2	57.6	53.3	347-1047 bp	1.0	52.0
trnR_3-groEL_p2	AATCCACTGAGCTACAAGCC	GCATCATCTTGATATAGAATTTGTT	50.0	28.0	57.6	54.1	347-1047 bp	1.0	52.0
trnR_3-groEL_p3	GCCATGTGCTCTAATCCACT	GCATCATCTTGATATAGAATTTGT	50.0	29.2	57.7	53.3	359-1059 bp	1.0	51.9
trnR_3-groEL_p4	GCCATGTGCTCTAATCCACT	GCATCATCTTGATATAGAATTTGTT	50.0	28.0	57.7	54.1	359-1059 bp	1.0	51.9
trnR_3-groEL_p5	GCCATGTGCTCTAATCCACT	GCATCATCTTGATATAGAATTTGTTT	50.0	26.9	57.7	54.8	359-1059 bp	1.0	51.9