Hotspot

trnP-ORF174

Genome · 4963-5019 · 57 bp

Back to Porphyra

Pi 0.111

MarkerSeek score 79.10

Primer available yes

Label trnP-ORF174

Feature Metadata

label_name: trnP-ORF174
feature_type: igs
region: Genome
length_bp: 57
variable_sites: 6
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-ORF174_p1	GCTACCAAGCTGCGCTATAT	GGGATGTTTTGGTATCCTAGA	50.0	42.9	57.9	54.4	102-105 bp	1.0	49.5
trnP-ORF174_p2	GCTACCAAGCTGCGCTATAT	GGGATGTTTTGGTATCCTAGAT	50.0	40.9	57.9	55.0	102-105 bp	1.0	47.0
trnP-ORF174_p3	TTTGAACCTGCGACATCCTG	GGGATGTTTTGGTATCCTAGA	50.0	42.9	58.5	54.4	138-141 bp	1.0	45.2
trnP-ORF174_p4	ATTTGAACCTGCGACATCCT	GGGATGTTTTGGTATCCTAGA	45.0	42.9	57.5	54.4	139-142 bp	1.0	45.1
trnP-ORF174_p5	CAGGATTTGAACCTGCGACA	GGGATGTTTTGGTATCCTAGA	50.0	42.9	58.5	54.4	143-146 bp	1.0	45.0