Hotspot

sufC

Genome · 14840-15601 · 762 bp

Back to Porosira

Pi 0.12

MarkerSeek score 79.50

Primer available yes

Label sufC

Feature Metadata

label_name: sufC
feature_type: gene
region: Genome
length_bp: 762
variable_sites: 91
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
sufC_p1	AAATGAGAAAGGTCAGGGCG	GCCTCCGCTAAGAATGTGAA	50.0	50.0	58.2	58.0	1563 bp	0.5	58.4
sufC_p2	AAATGAGAAAGGTCAGGGCG	TGCAAACTCTCCCTCTGGTA	50.0	50.0	58.2	58.0	1891 bp	0.5	58.4
sufC_p3	AAATGAGAAAGGTCAGGGCG	GAATCCGATCAGCTTCACGT	50.0	50.0	58.2	58.1	1697 bp	0.5	58.0
sufC_p4	AAATGAGAAAGGTCAGGGCG	ATGCACTAAGAAACCGGCAA	50.0	45.0	58.2	58.1	1612 bp	0.5	57.8
sufC_p5	AAATGAGAAAGGTCAGGGCG	TGCACTAAGAAACCGGCAAT	50.0	45.0	58.2	58.1	1611 bp	0.5	57.8