Hotspot

rps2

Genome · 29910-30635 · 726 bp

Back to Porosira

Pi 0.207

MarkerSeek score 83.20

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 726
variable_sites: 144
pi: 0.207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	AACAAAATCGCCCTTCTGGT	CACCATTACTACCACGCGAA	45.0	50.0	58.0	58.0	1797 bp	0.5	60.0
rps2_p2	AACAAAATCGCCCTTCTGGT	ACCCAAGCTTTGAATTCCGT	45.0	45.0	58.0	58.0	2118 bp	0.5	59.9
rps2_p3	CTCCAGTTGCAGCTGAAGAA	CACCATTACTACCACGCGAA	50.0	50.0	58.1	58.0	1497 bp	0.5	59.1
rps2_p4	TCCAGTTGCAGCTGAAGAAG	CACCATTACTACCACGCGAA	50.0	50.0	58.1	58.0	1496 bp	0.5	59.1
rps2_p5	TCCAGTTGCAGCTGAAGAAG	ACCCAAGCTTTGAATTCCGT	50.0	45.0	58.1	58.0	1817 bp	0.5	59.1