Hotspot

rps16

Genome · 86805-87038 · 234 bp

Back to Porosira

Pi 0.338

MarkerSeek score 72.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 234
variable_sites: 79
pi: 0.338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGCAAAGCTGGTACTAATGGA	CGTCGTGAAGGAGTAACTGG	42.9	55.0	57.3	58.0	1394 bp	0.5	46.1
rps16_p2	TGCAAAGCTGGTACTAATGGA	CCATGGCCACGTTACTGTAA	42.9	50.0	57.3	57.9	1278 bp	0.5	45.5
rps16_p3	TGCAAAGCTGGTACTAATGGA	CGGTATCGCGGACCTAAATT	42.9	50.0	57.3	57.9	1616 bp	0.5	45.1
rps16_p4	TGCAAAGCTGGTACTAATGGA	TTTATGTCACGGTATCGCGG	42.9	50.0	57.3	58.2	1625 bp	0.5	45.0
rps16_p5	TGCAAAGCTGGTACTAATGGA	CCGGTCACTCGCCCTATATA	42.9	55.0	57.3	58.2	1689 bp	0.5	44.9