Hotspot

rpl4

Genome · 93486-94133 · 648 bp

Back to Porosira

Pi 0.174

MarkerSeek score 79.70

Primer available yes

Label rpl4

Feature Metadata

label_name: rpl4
feature_type: gene
region: Genome
length_bp: 648
variable_sites: 112
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl4_p1	GAAACTTCGTTTGCTGTCGG	CGAATGAACAGTAGCGGGAA	50.0	50.0	58.0	58.0	1597 bp	0.5	59.8
rpl4_p2	GGGACGGTTCCAGAAAGATC	CGAATGAACAGTAGCGGGAA	55.0	50.0	58.0	58.0	1735 bp	0.5	59.8
rpl4_p3	CCAGGTTCGATTGGTATGGG	CGAATGAACAGTAGCGGGAA	55.0	50.0	58.0	58.0	1465 bp	0.5	59.7
rpl4_p4	GAAACTTCGTTTGCTGTCGG	TGATACTCCTGGCCCTGAAA	50.0	50.0	58.0	58.0	1726 bp	0.5	59.6
rpl4_p5	ACTCCAAAAGCGTTGGAACT	CGAATGAACAGTAGCGGGAA	45.0	50.0	57.9	58.0	1529 bp	0.5	59.5