Hotspot

rpl3

Genome · 92781-93437 · 657 bp

Back to Porosira

Pi 0.163

MarkerSeek score 83.80

Primer available yes

Label rpl3

Feature Metadata

label_name: rpl3
feature_type: gene
region: Genome
length_bp: 657
variable_sites: 102
pi: 0.163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl3_p1	ATTAATCACGGCTTGACGCT	AACACCACCACCTCTCCATA	45.0	50.0	58.0	58.0	1454 bp	0.5	59.5
rpl3_p2	ATTAATCACGGCTTGACGCT	ACACCACCACCTCTCCATAA	45.0	50.0	58.0	58.0	1453 bp	0.5	59.5
rpl3_p3	ATTAATCACGGCTTGACGCT	ATAACACCACCACCTCTCCA	45.0	50.0	58.0	58.0	1456 bp	0.5	59.5
rpl3_p4	ATTAATCACGGCTTGACGCT	TAACACCACCACCTCTCCAT	45.0	50.0	58.0	58.0	1455 bp	0.5	59.5
rpl3_p5	TCCGTAAAACTTGTGCGGAA	ACACCACCACCTCTCCATAA	45.0	50.0	58.0	58.0	1634 bp	0.5	59.3