Hotspot

rns-trnI-GAU

Genome · 71711-71766 · 56 bp

Back to Porosira

Pi 0.125

MarkerSeek score 84.00

Primer available yes

Label rns-trnI-GAU

Feature Metadata

label_name: rns-trnI-GAU
feature_type: igs
region: Genome
length_bp: 56
variable_sites: 7
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI-GAU_p1	GACTGGGGTGAAGTCGTAAC	CTGGGCCATTCTGGATTTGA	55.0	50.0	57.9	57.9	192 bp	1.0	81.1
rns-trnI-GAU_p2	GGAGGGTGCCTAAGGTAGAA	CTGGGCCATTCTGGATTTGA	55.0	50.0	58.1	57.9	217 bp	1.0	80.4
rns-trnI-GAU_p3	GGGGTGAAGTCGTAACAAGG	CTGGGCCATTCTGGATTTGA	55.0	50.0	58.2	57.9	188 bp	1.0	80.2
rns-trnI-GAU_p4	GTAACCGTACTGGAAGGTGC	CTGGGCCATTCTGGATTTGA	55.0	50.0	58.3	57.9	169 bp	1.0	79.9
rns-trnI-GAU_p5	TGACTGGGGTGAAGTCGTAA	CTGGGCCATTCTGGATTTGA	50.0	50.0	58.3	57.9	193 bp	1.0	79.4