Hotspot

trnT-UGU-ycf33

Genome · 52287-52563 · 277 bp

Back to Porosira

Pi 0.282
MarkerSeek score 74.10
Primer available yes
Label trnT-UGU-ycf33

Feature Metadata

label_name
trnT-UGU-ycf33
feature_type
igs
region
Genome
length_bp
277
variable_sites
64
pi
0.282
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-ycf33_p1 TGGTGTAGCTCAACGGTAGA CGACCTTGGGCTTATGAGTC 50.0 55.0 58.1 58.1 707-735 bp 1.0 90.8
trnT-UGU-ycf33_p2 GATTCCGGGTTTTCAGGGTT CGACCTTGGGCTTATGAGTC 50.0 55.0 58.1 58.1 1154 bp 0.5 58.9
trnT-UGU-ycf33_p3 TTGATTCCGGGTTTTCAGGG CGACCTTGGGCTTATGAGTC 50.0 55.0 58.1 58.1 1156 bp 0.5 58.9
trnT-UGU-ycf33_p4 GGCGATTCCCTAATTCTGGG CGACCTTGGGCTTATGAGTC 55.0 55.0 58.1 58.1 1318 bp 0.5 58.7
trnT-UGU-ycf33_p5 GATTCCGGGTTTTCAGGGTT TAGTAGATGGCTCTGGTGGG 50.0 55.0 58.1 57.9 1185 bp 0.5 58.6